Page 9

ACE Food & Beverage Applications Guide-Hichrom

Free Amino Acids in Peas (Pisum sativum) by HPLC-HRAM-MS Conditions Column: ACE 3 AQ Dimensions: 150 x 3.0 mm Part Number: ACE-116-1503 Mobile Phase: A: 0.1% formic acid in H2O B: 0.1% formic acid in MeCN Flow Rate: 0.4 mL/min Injection: 5 μL Temperature: 30 °C Detection: Exactive Orbitrap high resolution MS ESI positive ion mode Capillary temperature: 350 °C Application #AN2660 Time (mins) %B 0 0 10 100 100 80 60 40 100 80 60 40 20 100 Cys Gly Pro 0 1 2 3 4 5 6 7 8 9 10 11 12 Reproduced with permission of Fera Science Ltd, York, UK Relative Abundance Arg 100 80 60 40 20 0 His 100 80 60 40 20 0 Lys 100 80 60 40 20 0 Asp 100 80 60 40 20 0 100 80 60 40 20 0 100 80 60 40 20 0 100 80 60 40 20 0 Time (min) 80 60 40 20 0 100 80 60 40 20 0 100 80 60 40 20 0 Relative Abundance 100 80 60 40 20 0 100 80 60 40 20 0 100 80 60 40 20 0 100 80 60 40 20 0 100 80 60 40 20 0 100 80 60 40 20 0 100 Ser Asn Tyr Phe 0 1 2 3 4 5 6 7 8 9 10 11 12 Time (min) 80 60 40 20 0 20 Glu 0 0 Ileu Val Leu Ala Met Trp Gln Thr 7


ACE Food & Beverage Applications Guide-Hichrom
To see the actual publication please follow the link above