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ACE Food & Beverage Applications Guide-Hichrom

Water Soluble Vitamins in Green Vegetables by LC-MS/MS Conditions Column: ACE 3 C18 Dimensions: 100 x 2.1 mm Part Number: ACE-111-1002 Mobile Phase: A: 10 mM ammonium acetate in H2O, pH 4.5 B: 0.1% acetic acid in MeOH C: 0.3% acetic acid in MeOH Time (mins) %A %B %C 0 90 10 0 3 90 10 0 4 50 0 50 7 50 0 50 10 0 100 0 Flow Rate: 0.2 mL/min Injection: 10 μL Temperature: 20 °C Detection: TSQ triple quad MS; SRM mode -ESI for vitamin C +ESI for B vitamins Application #AN1860 Transition m/z 174.9 115.2 LOQ 128.1 ng/mL Transition m/z 265.1 122.1 LOQ 2.4 ng/mL Transition m/z 169.9 152.1 LOQ 0.6 ng/mL Transition m/z 123.0 80.3 LOQ 13.2 ng/mL Transition m/z 220.0 202.1 LOQ 23.3 ng/mL Transition m/z 180.1 105.2 Transition m/z 442.0 294.9 LOQ 1.9 ng/mL Transition m/z 377.1 243.0 LOQ 0.2 ng/mL 1 3 4 5 6 7 8 2 Reproduced with permission of Foodomics Laboratory, CIAL-CSIC, Madrid, Spain 87 1. Ascorbic acid 2. Thiamine 3. Pyridoxine 4. Nicotinamide 5. Pantothenic acid 6. Hippuric acid (IS) 7. Folic acid 8. Riboflavin


ACE Food & Beverage Applications Guide-Hichrom
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