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ACE Food & Beverage Applications Guide-Hichrom

Water Soluble Vitamins Conditions Column: ACE Ultracore 2.5 SuperPhenylHexyl Dimensions: 50 x 2.1 mm Part Number: CORE-25B-0502U Mobile Phase: A: 20 mM KH2PO4, pH 2.7 B: 20 mM KH2PO4, pH 2.7 in MeOH/H2O (50:50 v/v) Flow Rate: 0.4 mL/min Injection: 1 μL Temperature: 40 °C Detection: UV, 205 and 254 nm Application #AN1880 6 7 1 2 3 4 5 min 1 2 3 4 5 Time (mins) %B 0.00 20 1.50 60 3.00 70 3.75 70 4.50 20 Post time 4.5 minutes 86 1. Pyridoxine 2. Pantothenic acid 3. p-Aminobenzoic acid 4. Cyanocobalamin 5. d-Biotin 6. Folic acid 7. Riboflavin


ACE Food & Beverage Applications Guide-Hichrom
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