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ACE Food & Beverage Applications Guide-Hichrom

1. Pyridoxine (Vitamin B6) 2. Pantothenic acid (Vitamin B5) 3. p-Aminobenzoic acid 4. Folic acid (Vitamin B9/ Vitamin M) 5. D-Biotin (Vitamin B7/ Vitamin H) 6. Cyanocobalamin (Vitamin B12) 7. Riboflavin (Vitamin B2) Water Soluble Vitamins Conditions Column: ACE 3 C18-AR Dimensions: 150 x 4.6 mm Part Number: ACE-119-1546 Mobile Phase: A: 20 mM potassium phosphate pH 2.83 in H2O B: 20 mM potassium phosphate pH 2.83 in MeOH/H2O (50:50 v/v) Flow Rate: 1.5 mL/min Injection: 1 μL Temperature: 40 °C Detection: UV, 205 nm Application #AN1870 Time (mins) %B 0 20 15 70 0 2 4 6 8 10 12 14 16 min mAU 175 150 125 100 75 50 25 0 1 2 3 4 5 6 7 85


ACE Food & Beverage Applications Guide-Hichrom
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