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ACE Food & Beverage Applications Guide-Hichrom

Separation of Seven Water Soluble Vitamins using Isocratic Conditions (I) Application #AN2990 Conditions Column: ACE 5 C18 Dimensions: 250 x 4.6 mm Part Number: ACE-121-2546 Mobile Phase: 50 mM KH2PO4 pH 3.0 in H2O/MeOH (97:3 v/v) Flow Rate: 1 mL/min Temperature: Ambient Detection: UV, 205 nm 1. Pyridoxamine 2. Thiamine (Vitamin B1) 3. L-Ascorbic acid (Vitamin C) 4. Nicotinic acid 5. Pyridoxal 6. Impurity 7. Pyridoxine 1 2 3 4 5 6 7 83


ACE Food & Beverage Applications Guide-Hichrom
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