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ACE Food & Beverage Applications Guide-Hichrom

Separation of 13 Water Soluble Vitamins using a Gradient Conditions Column: ACE 5 C18 Dimensions: 250 x 4.6 mm Part Number: ACE-121-2546 Mobile Phase: A: 50 mM KH2PO4 pH 3.0 in H2O B: MeOH Flow Rate: 1 mL/min Temperature: Ambient Detection: UV, 205 nm Application #AN2930 Time (mins) %B 0 3 5 3 45 45 50 80 1. Pyridoxamine 2. Thiamine (Vitamin B1) 3. L-Ascorbic acid (Vitamin C) 4. Nicotinic acid 5. Pyridoxal 6. Impurity 7. Pyridoxine 8. p-Aminobenzoic acid 9. Pantothenic acid (Vitamin B5) 10. Folic acid (Vitamin B9) 11. Cyanocobalamin (Vitamin B12) 12. d-Biotin (Vitamin B7) 13. Riboflavin (Vitamin B2) 1 2 3 4 5 6 7 8 9 10/11 12 13 82


ACE Food & Beverage Applications Guide-Hichrom
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