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ACE Food & Beverage Applications Guide-Hichrom

Separation of 14 Water Soluble Vitamins Conditions Column: ACE 5 C8 Dimensions: 250 x 4.6 mm Part Number: ACE-122-2546 Mobile Phase: A: 50 mM KH2PO4 pH 2.5 in H2O B: MeOH Flow Rate: 1 mL/min Temperature: Ambient Detection: UV, 205 nm Application #AN2940 Time (mins) %B 0.0 0 3.0 0 16.5 45 19.5 80 1. Pyridoxamine 2. Thiamine (Vitamin B1) 3. L-Ascorbic acid (Vitamin C) 4. Niacinamide (Vitamin B3) 5. Nicotinic acid 6. Pyridoxal 7. Pyridoxine 8. p-Aminobenzoic acid 9. Pantothenic acid (Vitamin B5) 10. Folic acid (Vitamin B9) 11. Cyanocobalamin (Vitamin B12) 12. Riboflavin (Vitamin B2) 13. d-Biotin (Vitamin B7) 14. Thioctic acid 1 2 3 4 5 6 7 8 9 10 12 13 11 14 81


ACE Food & Beverage Applications Guide-Hichrom
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