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ACE Food & Beverage Applications Guide-Hichrom

Fast LC-MS Analysis of Multivitamin Fruit Juice Application #AN2610 Conditions Column: ACE Excel 3 C18-PFP Dimensions: 100 x 2.1 mm Part Number: EXL-1110-1002U Mobile Phase: A: 15 mM formic acid, adjusted to pH 3.8 with ammonia solution B: MeOH Time (mins) %B 0.00 1 1.00 1 3.00 8 3.10 25 6.00 50 6.50 50 6.51 1 9.00 1 Flow Rate: 0.4 mL/min Temperature: 30 °C Detection: LCMS-8040 triple quad MS ESI positive mode (ESI negative for ascorbic and citric acid) DL temperature: 250 °C Heart block temperature: 400 °C 1. Thiamine (Vitamin B1) (m/z 266.10 122.15) 2. Pyridoxine (Vitamin B6) (m/z 170.20 152.15) 3. Nicotinic acid (Vitamin B3) (m/z 124.00 78.00) 4. Pantothenic acid (Vitamin B5) (m/z 220.30 90.05) 5. Cyanocobalamin (Vitamin B12) (m/z 678.50147.05) 6. Riboflavin (Vitamin B2) (m/z 377.20 243.10) 7. Biotin (Vitamin B7) (m/z 245.10 227.05) 8. Nicotinamide (Vitamin B3) (m/z 123.20 80.05) 9. Ascorbic acid (Vitamin C) (m/z 175.10 114.80) 10. Citric acid (m/z 191.10 87.15) Standard 56 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 min Reproduced with permission of Shimadzu Europe GmbH 1 2 4 3 7 8 9 10 Juice sample 1 2 3 4 5 6 7 8 9 10 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 min 80


ACE Food & Beverage Applications Guide-Hichrom
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