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ACE Food & Beverage Applications Guide-Hichrom

25-Hydroxy Vitamin D in Serum by LC-MS/MS Conditions Column: ACE Excel 2 C18-PFP Dimensions: 100 x 2.1 mm Part Number: EXL-1010-1002U Mobile Phase: A: 2 mM ammonium acetate, 0.1% formic acid in H2O B: 0.1% formic acid in MeOH Time (mins) %B 0.0 75 3.0 100 4.0 100 Flow Rate: 0.4 mL/min Injection: 15 μL Temperature: 40 °C Detection: Quattro Premier XE triple quad MS MRM positive ESI mode Desolvation temperature: 450 °C Ion source temperature: 150 °C Application #AN2390 1a 1b 2 3a Reproduced with permission of Biotage GB Ltd 1a. 25-OH Vitamin D2 (m/z 395.5 269.5) 1b. 25-OH Vitamin D2 (m/z 395.5 119.2) 2. d6-25-OH Vitamin D3 (IS) (m/z 389.6 263.5) 3a. 25-OH Vitamin D3 (m/z 383.5 257.5) 3b. 25-OH Vitamin D3 (m/z 383.5 107.2) 3b 78


ACE Food & Beverage Applications Guide-Hichrom
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