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ACE Food & Beverage Applications Guide-Hichrom

Veterinary Steroids by LC-MS/MS Conditions Column: ACE UltraCore 2.5 SuperC18 Dimensions: 100 x 2.1 mm Part Number: CORE-25A-1002U Mobile Phase: A: 0.01 mM ammonium fluoride + 0.001% formic acid B: MeCN Time (mins) %B 0.0 25 0.5 25 7.0 35 7.5 35 10.5 60 12.5 90 Flow Rate: 0.5 mL/min Temperature: 45 °C Detection: Positive or negative ESI MRM data Application #AN1830 1. α- and β-Zearalenol (m/z 319.17 → 275.12) 2. Diethylstilbestrol-d8 (m/z 275.23 → 245.09) -ESI -ESI +ESI Reproduced with permission of The Food and Environment Research Agency, UK Also analysed in -ESI: Taleranol and zeranol-d4 Taleranol and zeranol Zearalenone Hexestrol Diethylstilbestrol Dienesterol 3. α- and β-Boldenone (m/z 287.17 → 121.12) Also analysed in +ESI: Hydroxystanozolol Hydroxystanozolol-d3 Methyltestosterone Methyltestosterone-d3 β-Nortestosterone-d3 β-Trenbolone α-Trenbolone 1β 1α 3α 3β 4α 4β 2 cis 2 trans +ESI 4. α- and β-Nortestosterone (m/z 275.23 → 109.09) 76


ACE Food & Beverage Applications Guide-Hichrom
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