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ACE Food & Beverage Applications Guide-Hichrom

Fast Separation of Vanillin Compounds Conditions Column: ACE Excel 1.7 C18-Amide Dimensions: 50 x 3.0 mm Part Number: EXL-1712-0503U Mobile Phase: A: 0.1% formic acid in H2O B: 0.1% formic acid in MeCN Flow Rate: 1.3 mL/min Injection: 1 μL Temperature: 45 °C Detection: UV, 260 nm Application #AN2240 1. Vanillic acid 2. 4-Hydroxybenzoic acid 3. Vanillin 4. 4-Hydroxybenzaldehyde Time (mins) %B 0.00 25 1.32 75 1.49 75 1.60 25 0.2 0.4 0.6 0.8 1 1.2 1.4 min mAU 250 200 150 100 50 0 1 2 3 4 5 6 7 8 5. Guaiacol 6. o-Vanillin 7. Ethyl vanillin 8. Eugenol 0 75


ACE Food & Beverage Applications Guide-Hichrom
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