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ACE Food & Beverage Applications Guide-Hichrom

Separation of Vanillins Conditions Column: ACE 3 C18-Amide Dimensions: 150 x 4.6 mm Part Number: EXL-1112-1546U Mobile Phase: A: 0.1% formic acid in H2O B: 0.1% formic acid in MeCN Time (mins) %B 0.0 30 10.0 55 10.5 55 15.0 30 Post time 5 minutes Flow Rate: 1 mL/min Injection: 5 μL Temperature: 40 °C Detection: UV, 260 nm Application #AN1620 1. Vanillic acid 2. 4-Hydroxybenzoic acid 3. Vanillin 4. 4-Hydroxybenzaldehyde 5. Guaiacol 6. Ethyl Vanillin 7.Eugenol 1 2 3 4 5 6 7 2 4 6 8 10 min 74


ACE Food & Beverage Applications Guide-Hichrom
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