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ACE Food & Beverage Applications Guide-Hichrom

Separation of Sulfonamides Conditions Column: ACE Excel 3 C18-PFP Dimensions: 150 x 4.6 mm Part Number: EXL-1110-1546U Mobile Phase: A: H2O B: MeCN C: 10% formic acid Flow Rate: 1 mL/min Detection: UV, 268 nm Application #AN1610 1. Sulfadiazine 2. Sulfapyridine 3. Sulfamerazine 4. Sulfamoxole 5. Sulfamethazine 6. Sulfamonomethoxine 7. Sulfachloropyridazine 8. Sulfamethoxazole 9. Sulfadimethoxine 1 4 3 2 5 6 7 8 9 2 4 6 8 10 12 14 16 mAU 40 30 25 20 15 10 5 mins 35 Time (mins) %A %B %C 0 84 15 1 12 74 25 1 14 59 40 1 16 84 15 1 18 84 15 1 Reproduced with permission of National Research Institute of Agriculture and Food Technology INIA, Spain 70


ACE Food & Beverage Applications Guide-Hichrom
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