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ACE Food & Beverage Applications Guide-Hichrom

Illegal Dyes in Spices Conditions Column: ACE 3 C18 Dimensions: 100 x 4.6 mm Part Number: ACE-111-1046 Mobile Phase: A: H2O B: MeOH C: 0.06 M Tetrabutylammonium bromide and 0.5 M KH2PO4 in H2O pH 2.55 Flow Rate: 1 mL/min Injection: 10 μL Temperature: Ambient Detection: UV-Vis, 420 nm, 520 nm and 600 nm Application #AN2910 1. Rhodamine B 2. Orange II 3. Metanil Yellow 4. Butter Yellow 5. Para Red 6. Sudan Orange G 7. Toluidine Red 8. Sudan I 9. Sudan Red G 10. Sudan II 11. Sudan Black 12. Sudan III 13. Sudan Red 7B 14. Sudan Red B 15. Sudan IV Time (mins) %A %B %C 0 45 50 5 45 3 92 5 65 3 92 5 66 45 50 5 75 45 50 5 1 2 3 4 5 7/8 6 14 9 10 12 11 13 15 Reproduced with permission of Lincolne Sutton & Wood, Norwich, UK 69


ACE Food & Beverage Applications Guide-Hichrom
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