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ACE Food & Beverage Applications Guide-Hichrom

Application #AN1390 Sennoside A Sennoside B Sennosides in Traditional Chinese Medicine Conditions Column: ACE 3 C18-PFP Dimensions: 150 x 4.6 mm Part Number: ACE-1110-1546 Mobile Phase: A: 0.75% acetic acid in H2O B: MeCN/MeOH (90:10 v/v) Time (mins) %B 0 9 23 28 40 28 Flow Rate: 0.6 mL/min Temperature: 35 °C Detection: UV, 271 nm Sample: Herbal tea bag containing Folium Sennae, Peppermint, Folium Mori, Folium Nelumbinis, Glycyrrhiza Uralensis and Lelang Grass Rhizome 1,000 800 600 400 200 -200 -400 0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 0 mAU min Absorbance mAU Retention Time min 2 1 Sennoside B Sennoside A Sample Standard Reproduced with permission of Vercotech Inc., Taiwan 68


ACE Food & Beverage Applications Guide-Hichrom
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