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ACE Food & Beverage Applications Guide-Hichrom

LC-MS/MS of Phytoestrogens from Hop Extract Conditions Column: ACE 3 C18-AR Dimensions: 150 x 4.6 mm Part Number: ACE-119-1546 Mobile Phase: A: 1% formic acid in MeCN B: 1% formic acid in MeOH C: 1% formic acid in H2O D: MeOH Time (mins) %A %B %C %D 0 56 0 44 0 8 51 5 44 0 10 51 5 44 0 17 95 5 0 0 22 95 0 0 5 Flow Rate: 0.6 mL/min Detection: TSQ-Quantum triple quad ESI Spray voltage: -4500 V Precursor ion: 355.4 M+H+ MRM transition ions: 179 and 299 Collision energy: 28 and 16 V Application #AN1160 1 2 2 4 6 8 10 12 14 16 Time (min) 28e+5 26e+5 24e+5 22e+5 18e+5 16e+5 14e+5 12e+5 10e+5 8e+5 6e+5 4e+5 2e+5 0 Intensity 20e+5 Reproduced with permission of Institute of Food Science Research, University of Madrid, Spain 65 1. Isoxanthohumol LOQ 0.07 μg/mL 2. Xanthohumol LOQ 0.01 μg/mL


ACE Food & Beverage Applications Guide-Hichrom
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