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ACE Food & Beverage Applications Guide-Hichrom

Perfluoro Acids by LC-MS/MS Conditions Column: ACE Excel 2 C18 Dimensions: 50 x 2.1 mm Part Number: EXL-101-0502U Mobile Phase: A: 2 mM ammonium acetate, 0.1% acetic acid/MeCN (95:5 v/v) B: 2 mM ammonium acetate, 0.1% acetic acid/MeCN (5:95 v/v) Flow Rate: 0.5 mL/min Injection: 20 μL Temperature: 40 °C Detection: AB SCIEX triple quad 5500 Negative ESI MRM Source temperature: 450 °C IonSpray voltage: -2400 V Application #AN1280 1. Heptafluorobutyric acid (m/z 212.9 → 168.9) 2. Perfluorohexanoic acid (m/z 313 → 268.9) 3. Perfluorobutylsulfonic acid (m/z 299 → 79.9) 4. Perfluoroheptanoic acid (m/z 363 → 319) 5. Perfluorooctanoic acid (m/z 413 → 368.9) 6. Perfluorohexylsulfonic acid (m/z 399 → 80) 7. Perfluorononanoic acid (m/z 463 → 419) 8. Perfluorodecanoic acid (m/z 513 → 469) 9. Perfluorooctanesulfonic acid (m/z 499 → 80) Time (mins) %B 0.0 25 0.5 25 5.5 95 7.5 95 8.0 25 10.0 25 1 2 4 5 7 8 6 3 9 Reproduced with permission of Biotage GB Ltd, UK 53


ACE Food & Beverage Applications Guide-Hichrom
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