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ACE Food & Beverage Applications Guide-Hichrom

Fast Separation of Organic Acids Conditions Column: ACE 3 C18-Amide ACE Excel 1.7 C18-Amide Dimensions: 250 x 2.1 mm 50 x 3.0 mm Part Number: 250 x 2.1 mm (EXL-1112-2502U), 50 x 3 mm (EXL-1712-0503U) Mobile Phase: 20 mM H3PO4 in MeOH/H2O (40:60 v/v) Flow Rate: 0.21 mL/min (250 x 2.1 mm) 0.8 mL/min (50 x 3.0 mm) Injection: 5 μL (250 x 2.1 mm) 2 μL (50 x 3.0 mm) Temperature: 20 °C Detection: UV, 210 nm Application #AN2200 ACE 3 C18-Amide 5 10 15 20 25 30 35 40 min mAU 200 175 150 125 100 75 50 25 0 1 2 3 4 5 mAU 175 150 125 100 75 50 25 0 1 2 3 4 5 ACE Excel 1.7 C18-Amide 5 10 15 20 25 30 35 40 min I 0 I 0 48 1. Sinapic acid 2. Ferulic acid 3. 3,4-Dimethoxycinnamic acid 4. Cinnamic acid 5. 4-Methoxycinnamic acid


ACE Food & Beverage Applications Guide-Hichrom
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