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ACE Food & Beverage Applications Guide-Hichrom

Separation of Milk Proteins Conditions Column: ACE 5 C18-300 Dimensions: 150 x 2.1 mm Part Number: ACE-221-1502 Mobile Phase: A: 0.01% TFA in H2O B: 0.01% TFA in MeCN Flow Rate: 0.2 mL/min Temperature: 45 °C Detection: UV, 214 nm Application #AN1540 Time (mins) %B 0.0 33 5.0 33 9.0 35 18.0 37 22.0 40 27.5 41 28.0 41 43.0 43 mAU 125 100 75 50 25 0 MW ~18 kDa MW ~24 kDa β lactoglobulins β caseins MW ~23-25 kDa α caseins 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 32.5 Time min 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 32.5 mins Reproduced with permission of The Chemical Analysis Facility, University of Reading, UK 41


ACE Food & Beverage Applications Guide-Hichrom
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