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ACE Food & Beverage Applications Guide-Hichrom

Microcystins from Blue/Green Algae in Drinking Water Conditions Column: ACE Excel 2 C18 Dimensions: 100 x 2.1 mm Part Number: EXL-101-1002U Mobile Phase: A: 0.1% formic acid in H2O B: MeCN Flow Rate: 0.4 mL/min Injection: 50 μL Temperature: 40 °C Sample: 0.05 ppb Detection: Bruker EVOQ Elite triple quad MS VIP heated-ESI temperature: 350 °C Cone gas temperature: 200 °C Spray voltage: 4500 V (+) Collision gas: argon 1.5 mTorr Application #AN1190 1. Microcystin RR (MW 1038) (m/z 520 → 135) 2. Microcystin LR (MW 995) (m/z 498 → 135) 3. Microcystin YR (MW 1045) m/z 523 → 135) Time (mins) %B 0.0 30 1.0 30 7.0 95 7.1 30 10.0 30 1 1 2 3 TIC 2 3 Reproduced with permission of Bruker UK Ltd Variants R L Microcystin-LR Leucine Arginine Microcystin-RR Arginine Arginine Microcystin-YR Tyrosine Arginine 40


ACE Food & Beverage Applications Guide-Hichrom
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