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ACE Food & Beverage Applications Guide-Hichrom

Fingerprinting of Liquorice Extracts Conditions Column: ACE 3 C18-PFP Dimensions: 150 x 2.1 mm Part Number: ACE-1110-1502 Mobile Phase: A: Ammonium acetate in H2O pH 4 B: MeOH Application #AN2090 Time (mins) %B 0 10 1 10 11 15 55 90 60 100 Flow Rate: 0.4 mL/min Injection: 2 μL Temperature: 40 °C Detection: UV, 260, 280, 330 and 350 nm Sample: Plant material ground to a fine powder in pestle and mortar. Powdered material extracted into methanol by ultrasonification for 30 minutes, followed by centrifugal filtration. Methanolic liquorice leaf extract at different wavelengths 260 nm 280 nm 330 nm 350 nm Liquorice leaf Liquorice root Comparison of methanolic extracts at 260 nm Reproduced with permission of Unilever, Colworth Science Park, UK 37


ACE Food & Beverage Applications Guide-Hichrom
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