Page 33

ACE Food & Beverage Applications Guide-Hichrom

Metabolite Profiling of Green Tea by LC-MS Conditions Column: ACE Excel 1.7 C18-Amide Dimensions: 100 x 2.1 mm Part Number: EXL-1712-1002U Mobile Phase: A: 0.01% formic acid in H2O B: 0.01% formic acid in MeCN Time (mins) %B 0.0 3 2.5 10 8.0 100 8.5 3 10.0 3 Flow Rate: 0.5 mL/min Detection: Exactive accurate mass MS system ESI in negative ion mode Analytes between m/z 70-800 monitored Application #AN2580 Sample: Metabolites from green tea extracted into cold water by vortexing for 20 mins. Samples filtered prior to injection onto column and modular Accela LC system Reproduced with permission of School of Pharmacy, University of Nottingham, UK 31


ACE Food & Beverage Applications Guide-Hichrom
To see the actual publication please follow the link above