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ACE Food & Beverage Applications Guide-Hichrom

Detection of Flavone and Dibucaine Conditions Column: ACE 3 C18 Dimensions: 30 x 4.6 mm Part Number: ACE-111-0346 Mobile Phase: A: 6.5 mM ammonium acetate in H2O B: MeCN C: MeOH Time (mins) %A %B %C 0.0 80 10 10 5.2 0 50 50 5.6 0 0 100 Flow Rate: 2 mL/min Temperature: 60 °C Detection: UV, 200-450 nm Application #AN2850 1. Flavone 2. Dibucaine 1 2 Reproduced with permission of Johnson & Johnson Pharmaceutical Research & Early Development, a division of Janssen-Cilag, S.A, Toledo, Spain 27


ACE Food & Beverage Applications Guide-Hichrom
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