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ACE Food & Beverage Applications Guide-Hichrom

Human Defensins in a Saliva Matrix Conditions Column: ACE UltraCore 2.5 SuperC18 Dimensions: 50 x 3.0 mm Part Number: CORE-25A-1503U Mobile Phase: A: 0.1% formic acid in H2O B: 0.1% formic acid in MeCN Flow Rate: 0.6 mL/min Sample Preparation: SPE on C18 Detection: Synapt G1 QToF +ESI MS Sampling cone voltage: 40 V Source temperature: 150 °C Capillary voltages: 4.8 kV Extraction cone voltages: 41 kV Desolvation temperature: 500 °C Acquisition: 100-2000 m/z Application #AN1270 Extracted ion current chromatograms (sum of multiply protonated ions M+3H3+, M+4H4+ and M+5H5+) 1 Time (mins) %B 0 2 2 2 17 50 19 95 20 95 TIC Area of interest Reproduced with permission of School of Science, University of Greenwich, UK Mass spectra 2 3 1 2 3 Defensin Human Neutrophil Peptides 1. HNP-1 (30 amino acid residues) 2. HNP-2 (29 amino acid residues) 3. HNP-3 (30 amino acid residues) 25


ACE Food & Beverage Applications Guide-Hichrom
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