Page 24

ACE Food & Beverage Applications Guide-Hichrom

Chocolate Analysis Conditions Column: ACE Excel 2 C18-Amide Dimensions: 100 x 2.1 mm Part Number: EXL-1012-1002U Mobile Phase: A: 10 mM ammonium formate pH 2.8 in H2O B: 10 mM ammonium formate pH 2.8 in MeCN/H2O (90:10 v/v) Flow Rate: 1.2 mL/min Temperature: 42 °C Detection: UV, 254 nm Application #AN2040 1. Acesulfame K 2. Theobromine 3. Saccharin 4. Theophylline 5. Caffeine 6. Chlorogenic acid 7. Catechin 8. Epicatechin 9. 4-Hydroxybenzoic acid 10. Vanillin 11. Guaiacol 12. Sorbic acid 13. Ethylvanillin 14. Methyl paraben 15. Ethyl paraben 16. Quercetin 17. Propyl paraben Time (mins) %B 0.0 5 1.5 85 mAU 350 300 250 200 150 100 12 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5 50 0 1 2 3 4 5 6 7 8 9 10 11 13 14 15 16 17 min Reproduced with permission of Shimadzu Europa GmbH. Analysed on Shimadzu Nexera X2. 22


ACE Food & Beverage Applications Guide-Hichrom
To see the actual publication please follow the link above