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ACE Food & Beverage Applications Guide-Hichrom

Chloramphenicol in Milk by LC-MS/MS Conditions Column: ACE 3 C18 Dimensions: 50 x 2.1 mm Part Number: ACE-111-0502 Mobile Phase: A: H2O B: MeOH Flow Rate: 0.5 mL/min Injection: 10 μL Detection: Bruker EVOQ Elite triple quad MS VIP heated-ESI temperature: 400 °C Cone gas temperature: 350 °C Spray voltage: -4500 V Application #AN2030 Time (mins) %B 0.00 10 0.05 10 2.50 95 3.00 95 3.10 10 4.50 10 TIC of 3 MRMs of 0.02 ppb chloramphenicol spiked in milk matrix MRM chromatograms of 0.05 ppb chloramphenicol in milk Milk Matrix Blank Reproduced with permission of Bruker UK Ltd CAP (0.02 ppb in milk matrix) Chloramphenicol m/z 320.9 → 152 Quantification m/z 320.9 → 257 Qualification m/z 320.9 → 194 Qualification 21


ACE Food & Beverage Applications Guide-Hichrom
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