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ACE Food & Beverage Applications Guide-Hichrom

Caffeine and Metabolites Conditions Column: ACE 5 SuperC18 Dimensions: 150 x 4.6 mm Part Number: EXL-1211-1546U Mobile Phase: A: 20 mM ammonium acetate pH 7.0 in H2O B: 20 mM ammonium acetate pH 7.0 in MeCN/H2O (90:10 v/v) Flow Rate: 1 mL/min Injection: 1 μL Temperature: 60 °C Detection: UV, 273 nm Application #AN2010 1. Xanthine 2. Theobromine 3. Paraxanthine 4. Theophylline 5 10 15 20 25 30 min 1 2 3 4 5 Time (mins) %B 0 2 45 15 48 15 49 2 Post time 10 minutes 5. Caffeine 0 5 10 15 20 25 30 mmiinnss 20


ACE Food & Beverage Applications Guide-Hichrom
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