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ACE Food & Beverage Applications Guide-Hichrom

Artificial Sweeteners (Stevia Glycosides) Conditions Column: ACE Excel 2 SuperC18 Dimensions: 150 x 2.1 mm Part Number: EXL-1011-1202U Mobile Phase: A: 10 mM sodium dihydrogen phosphate pH 2.8 in H2O B: 10 mM sodium dihydrogen phosphate pH 2.8 in H2O/MeCN (80:20 v/v) Flow Rate: 0.6 mL/min Injection: 1 μL Temperature: 50 °C Detection: UV, 200 nm Application #AN1020 1. Rebaudioside D 2. Rebaudioside A 3. Stevioside 4. Rebaudioside F 5. Rebaudioside C 6. Dulcoside A 7. Rubusoside 8. Rebaudioside B 9. Steviolbioside Time (mins) %B 0 39.5 4 48.0 1 2 3 4 5 6 Stevioside m/z = 804.87 7 8 9 Reproduced with permission of Shimadzu Europe GmbH. Analysed on Shimadzu Nexera X2. http://images2.advanstar.com/PixelMags/lcgc-eu/pdf/2013-10-sp.pdf Rebaudioside m/z = 967.01 17


ACE Food & Beverage Applications Guide-Hichrom
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