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ACE Food & Beverage Applications Guide-Hichrom

Separation of Artificial Food Colours Conditions Column: ACE 3 C18 Dimensions: 100 x 4.6 mm Part Number: ACE-111-1046 Mobile Phase: A: 3.1 mM tetrabutylammonium bromide and 5 mM KH2PO4 in H2O B: 5 mM KH2PO4 in MeOH Time (mins) %B 0 45 12 60 25 45 Flow Rate: 0.8 mL/min Injection: 10 μL Temperature: Ambient Detection: UV-Vis, 480 nm Application #AN2960 1. Tartrazine 2. Amaranth 3. Sunset Yellow 4. Ponceau 4R 1 2 3 4 Reproduced with permission of Lincolne Sutton & Wood, Norwich, UK 15


ACE Food & Beverage Applications Guide-Hichrom
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