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ACE Food & Beverage Applications Guide-Hichrom

Arsenolipids from Edible Seaweed by LC-ICP-MS and LC-ESI-MS Conditions Column: ACE 3 C18 Dimensions: 150 x 4.6 mm Part Number: ACE-111-1546 Mobile Phase: A: 0.1% formic acid in H2O B: 0.1% formic acid in MeOH Time (mins) %B 0 0 20 100 45 100 Flow Rate: 1 mL/min Injection: 100 μL Temperature: 45 °C Detection: Split ratio: 75% ESI-MS: 25% ICP-MS Thermo Scientific Element 2 ICP-MS Mode: Organic mode Medium resolution Thermo Scientific Orbitrap Discovery Positive ESI mode Spray voltage: 4.5 kV Capillary temperature: 320 °C Capillary voltage: 42 V Arsenic-containing hydrocarbon: Arsenic-containing phospholipids: Application #AN1970 m/z 389 M + H+ for C21H46AsO m/z 959 M + H+ for C45H89AsO14P (C16:0/C16:0) m/z 987 M + H+ for C47H93AsO14P (C18:0/C16:0) m/z 1015 M + H+ for C49H97AsO14P (C20:0/C16:0) 2.E+07 1.E+07 5.E+06 0.E+00 -5.E+06 ESI-MS Reproduced with permission of Trace Element Speciation Laboratory, University of Aberdeen, UK 10000000 9000000 8000000 7000000 6000000 5000000 4000000 3000000 2000000 1000000 0 -1.E+07 0 5 10 15 20 25 30 35 40 45 50 relative intensity ICP-MS relative intensity (ES-MS) 389 959 987 1015 As ICP-MS m/z 75 13


ACE Food & Beverage Applications Guide-Hichrom
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