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ACE Food & Beverage Applications Guide-Hichrom

Aminoglycosides in Eggs Conditions Column: ACE Excel 2 C18-PFP Dimensions: 100 x 2.1 mm Part Number: EXL-1010-1002U Mobile Phase: A: 20 mM HFBA in H2O/MeCN (98:2 v/v) B: 20 mM HFBA in MeCN/H2O (98:2 v/v) Flow Rate: 0.4 mL/min Temperature: 40 °C Detection: Positive ESI MRM (transitions as shown) Sample: Extraction at low pH, clean up with WCX SPE cartridge Egg sample spiked at 100 μg/kg (CCα) Application #AN1920 Time (mins) %B Curve 0.0 5.0 - 2.0 15.0 6 4.5 19.0 6 5.5 19.5 8 6.0 22.0 6 7.0 35.0 6 9.0 48.0 8 9.5 5.0 6 Key GNT Gentamicin TBM Tobramycin KNM Kanamycin DHS Dihydrostreptomycin STR Streptomycin HGB Higromycin-B SPT Spectinomycin Reproduced with permission of Veterinary Drug Residues Department, Centro Nacional de Alimentación (CNA, AECOSAN), Madrid, Spain 9


ACE Food & Beverage Applications Guide-Hichrom
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