ACE UHPLC and HPLC Columns
Time - Minutes
Malonic acid 4-Hydroxybutyric acid 3-Hydroxybutyric acid
3-Hydroxyisobutyric acid 2-Hydroxybutyric acid
100%
EIC
Reproduced with permission of UCSD Biochemical Genetics, Department of Pediatrics, University of California San Diego, California, USA
5
2b
3
2a 9
100%
EIC
0
14 18 22 26 30 34
Time - Minutes
1
4
6
7
8
1. Malonic acid
(m/z 103 59)
2. 4-Hydroxybutyric acid
(m/z 103 57)
3. 3-Hydroxybutyric acid
(m/z 103 59)
4. 3-Hydroxyisobutyric acid
(m/z 103 73)
5. 2-Hydroxybutyric acid
(m/z 103 57)
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com 99
Metabolomics – C4 Hydroxy Acids
Metabolomics – C6 & C7 Hydroxy and Dicarboxylic Acids
Application #AN4120
Application #AN4100
Conditions
Conditions
Analytes
Analytes
1. 5-Hydroxyhexanoic acid
(m/z 131 85)
2. 2-Hydroxy-3-methylvaleric acid
(m/z 131 73)
3. 3-Methylglutaric acid
(m/z 145 101)
4. Adipic acid
(m/z 145 83)
5. 2-Hydroxyisocaproic acid
(m/z 131 85)
6. 3-Methyladipic acid
(m/z 159 115)
7. Pimelic acid
(m/z 159 97)
8. 4-Hydroxyphenylacetic acid
(m/z 151 107)
9. 2-Hydroxyphenylacetic acid
(m/z 151 107)
Column: ACE 3 C18-PFP
Dimensions: 150 x 2.1 mm
Part Number: ACE-1110-1502
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Detection: Sciex API 4000 triple quad MS
ESI in negative ion mode
Sample: Urine / plasma sample is filtered and
extracted with ice-cold methanol,
evaporated to dryness and reconstituted
in 0.1% formic acid in water
Please contact info@ace-hplc.com for additional information
on the chromatographic conditions used for this analysis.
Column: ACE 3 C18-PFP
Dimensions: 150 x 2.1 mm
Part Number: ACE-1110-1502
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Detection: Sciex API 4000 triple quad MS
ESI in negative ion mode
Sample: Urine / plasma samples filtered and
extracted with ice-cold methanol,
evaporated to dryness and reconstituted
in 0.1% formic acid in water.
Please contact info@ace-hplc.com for additional information
on the chromatographic conditions used for this analysis.
1 2
3
4
5
0.0
0
1.0 2.0 3.0 4.0 5.0 6.0 7.0
Reproduced with permission of UCSD Biochemical Genetics, Department of Pediatrics, University of California San Diego, California, USA