ACE UHPLC and HPLC Columns
Metabolomics and Biochemical Genetics - Acylglycines
100%
Reproduced with permission of UCSD Biochemical Genetics, Department of Pediatrics, University of California San Diego, California, USA
Metabolomics – C4 & C5 Hydroxy and Dicarboxylic Acids
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www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com
11
11
12a
EIC
1
12b
12b
13
10
15
16
5
4
2
10
10
9
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8
2
3
5
3 6
14
4
3
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Column: ACE 3 C18-PFP
Dimensions: 150 x 2.1 mm
Part Number: ACE-1110-1502
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Detection: Sciex API 4000 triple quad MS
ESI in negative ion mode
Sample: Urine / plasma sample is filtered and
extracted with ice-cold methanol, evaporated
to dryness and reconstituted in 0.1% formic acid in water
Please contact info@ace-hplc.com for additional information on the
chromatographic conditions used for this analysis.
Column: ACE 3 C18-PFP
Dimensions: 150 x 2.1 mm
Part Number: ACE-1110-1502
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Detection: Sciex API 4000 triple quad MS
ESI in negative ion mode
Sample: Urine / plasma sample is filtered
and extracted with ice-cold
methanol, evaporated to dryness
and reconstituted in 0.1% formic
acid in water
Please contact info@ace-hplc.com for
additional information on the chromatographic
conditions used for this analysis.
Application #AN4080
Application #AN4110
Conditions
Conditions
Analytes
Analytes
1. Propionylglycine
(m/z 130 74)
2. Isobutyrylglycine
(m/z 144 74)
3. Butyrylglycine
(m/z 144 74)
4. 2-Methylbutyrylglycine
(m/z 158 74)
5. Isovalerylglycine
(m/z 158 74)
6. Tiglylglycine
(m/z 156 112)
7. Valerylglycine
(m/z 158 74)
8. 3-Methylcrotonylglycine
(m/z 156 74)
9. Suberylglycine
(m/z 230 74)
10. Hexanoylglycine
(m/z 172 74)
11. Trans-Cinnamoylglycine
(m/z 204 160)
1. Isocitric acid
(m/z 191 111)
2. 2-Hydroxyglutaric acid
(m/z 147 129)
3. 3-Hydroxyglutaric acid
(m/z 147 85)
4. Maleic acid
(m/z 115 71)
5. Citric acid
(m/z 191 111)
6. Fumaric acid
(m/z 115 71)
7. Succinic acid
(m/z 117 73)
8. Methylmalonic acid
(m/z 117 55)
9. 3-Hydroxy-3-methylglutaric acid
(m/z 161 99)
10. 2-Hydroxyadipic acid
(m/z 161 143)
11. 3-Hydroxyisovaleric acid
(m/z 117 59)
12. 3-Hydroxy-2-methylbutyric acid
(m/z 117 73)
13. Glutaric acid
(m/z 131 87)
14. 2-Ethyl-3-hydroxypropionic acid
(m/z 117 87)
15. Ethylmalonic acid
(m/z 131 87)
16. Methylsuccinic acid
(m/z 131 87)
Time - Minutes
6
7
8
9
12a
4.0 6.0 8.0 10.0 12.0 14.0
Time - Minutes
4
6
11
10
0
0
8 12 16 20 24 28 32 36
1
2.0
Reproduced with permission of UCSD Biochemical Genetics, Department of Pediatrics, University of California San Diego, California, USA
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