ACE UHPLC and HPLC Columns
Lubricant Additives: ADPA/OPNA Antioxidants
Application #AN1170
mAU
1200
1000
800
600
400
200
C4-ADPA C8-ADPA C4-OPNA
1
2
3
4
5
Conditions Analytes
Time - Minutes
7.0e4
6.0e4
5.0e4
4.0e4
3.0e4
2.0e4
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Conditions Analytes
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: CORE-25A-1546U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in
MeCN/IPA (1:2 v/v)
Gradient: Time (mins) %B
0.0 65.0
15.0 97.5
25.0 97.5
25.1 65.0
Flow Rate: 1 mL/min
Temperature: 60 °C
Detection: UV, 220 nm
1. C4-ADPA
2. C8-ADPA
3. C4-OPNA
4. C12-ADPA
5. C16-ADPA
0
0.0 2.5 5.0 7.5 10 12.5 15.0 17.5 20.0 22.5
C16-ADPA C12-ADPA
Reproduced with permission of Formulated Product Technology, BP Technology Centre, UK
Lurbinectedin in Plasma by LC-MS/MS
Application #AN3810
Column: ACE 3 C18-PFP
Dimensions: 30 x 2.1 mm
Part Number: ACE-1110-0302
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0.0 10
2.5 90
3.5 90
3.6 10
5.0 10
Flow Rate: 0.6 mL/min
Injection: 5 μL
Temperature: 50 °C
Detection: API 4000 triple quad
TurbolonSpray, ESI positive ion mode
Turbo Temperature: 650 °C
Ion Spray Potential: 5000 V
1. Lurbinectedin
(m/z 767.3 273.0)
(LLOQ 0.1 ng/mL)
2. d4-Lurbinectedin (IS)
(m/z 771.4 277.0)
Lurbinectedin d4-Lurbinectedin (IS)
1
2
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
Time - Minutes
1.0e4
Intensity (cps)
Reproduced with permission of PharmaMar S.A., Madrid, Spain