ACE UHPLC and HPLC Columns
Lipid Classes Separation from Drosophila Melanogaster
2 5ii
100
TIC +ve ion
TIC -ve ion
nm
280 nm
330 nm
350 nm
Liquorice leaf
Liquorice root
Methanolic liquorice leaf extract
at different wavelengths
Comparison of methanolic
extracts at 260 nm
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Column: ACE 3 SIL
Dimensions: 150 x 3.0 mm
Part Number: ACE-117-1503
Mobile Phase: A: IPA/MeCN (20:80 v/v)
B: IPA/0.02 M ammonium formate
(20:80 v/v)
Gradient: Time (mins) %B
0.0 8
1.0 8
5.0 9
10.0 20
16.0 25
23.0 35
26.0 8
Flow Rate: 0.3 mL/min
Detection: LTQ Orbitrap MS
Positive and negative ion mode
Application #AN1530
Conditions Analytes
1. Triglyceride (TG)
2. Phosphoserine (PS)
3i. Phosphoethanolamine (PE)
3ii. Lyso phosphoethanolamine (Lyso PE)
4. Sphingomyelin phosphoethanolamine (SMPE)
5i. Phosphatidylcholine (PC)
5ii. Lyso phosphatidylcholine (Lyso PC)
6. Glycerophosphoglycerol (GPG)
7. Phosphoinositol (PI)
1
2
3i
4
5i
6
7
5i
5ii
3i
3ii
1
2
3i
4
3ii
5i
5ii
5i
5ii
3i
2
7
6
100
Relative abundance
2 4 6 8 10 12 14 16 18 20 22 24 26 28
Reproduced with permission of University of Strathclyde, Glasgow, UK
Liquorice Extracts Fingerprint
Column: ACE 3 C18-PFP
Dimensions: 150 x 2.1 mm
Part Number: ACE-1110-1502
Mobile Phase: A: Ammonium acetate in H2O pH 4
B: MeOH
Gradient: Time (mins) %B
0 10
1 10
11 15
55 90
60 100
Flow Rate: 0.4 mL/min
Injection: 2 μL
Temperature: 40 °C
Detection: UV, 260, 280, 330 and 350 nm
Sample: Plant material ground to a fine
powder in pestle and mortar.
Powdered material extracted
into methanol by ultrasonification
for 30 minutes, followed by
centrifugal filtration.
Application #AN2090
Conditions
260 nm
280 nm
330 nm
nm
Liquorice leaf
Liquorice root
0 10 20 30 40 50
Reproduced with permission of Unilever, Colworth Science Park, UK
Time - Minutes
Time - Minutes
Drosophila Melanogaster