ACE UHPLC and HPLC Columns
Lidocaine in Saliva by LC-MS/MS
Column: ACE 3 C18
Dimensions: 100 x 3.0 mm
Part Number: ACE-111-1003
Mobile Phase: A: 0.1% formic acid in
MeCN/H2O (20:80 v/v)
B: 0.1% formic acid in
MeCN/H2O (80:20 v/v)
Gradient: Time (mins) %B
0.0 20
1.0 20
3.0 80
4.5 80
Flow Rate: 0.3 mL/min
Injection: 10 μL
Detection: Quattro-Micro triple quad MS
Positive ion mode ESI
Saliva samples taken after “Emla 5 %” application to skin
Reproduced with permission of Department of Analytical Chemistry, Stockholm University, Sweden
Lincosamide Antibiotics
150
100
50
92
1
2
100
%
100
%
Clindamycin phosphate
0 1 2 3 4 5 6 7 8
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0
Application #AN2570
Conditions Analyte
1. Lidocaine
(m/z 235 86)
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: 0.02 M sodium phosphate
dibasic pH 3.0/MeCN (70:30 v/v)
Flow Rate: 1 mL/min
Injection: 25 μL
Temperature: 25 °C
Detection: UV, 205 nm
Application #AN2650
Conditions Analytes
1. Lincomycin HCl
2. Clindamycin phosphate
Patient saliva sample
1. Lidocaine
Blank saliva
Patient saliva sample
1
0
1.00 2.00 3.00 4.00 5.00
0
1.00 2.00 3.00 4.00 5.00
Lincomycin HCl
Reproduced with permission of Department of Pharmacy, Faculty of
Medicine and Surgery, University of Malta
Time - Minutes
Time - Minutes
mAU
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