ACE UHPLC and HPLC Columns
Lansoprazole and Degradation Products after Acidic Hydrolysis in 0.1 M HCl
Conditions Analyte
Column: ACE Excel 5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: EXL -1211-1546U
Mobile Phase: A: 0.1% ammonia in H2O
B: 0.1% ammonia in MeCN/H2O (90:10 v/v)
Gradient: Time 1 (mins) Time 2 (mins) Time 3 (mins) %B
0.0 0.0 0.0 10
15.0 30.0 45.0 90
15.5 30.5 45.5 90
18.0 33.0 48.0 10
Post time 10 minutes
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 30 °C
Detection: UV, 280 nm
Application #AN1520
1. Lansoprazole
1
Lansoprazole
5 10 20 25 30 35 40 45
5 10 15 20 35 45 min
1
1
5 10 15 20 25 30 35 40
45 min
5 10 20 25 35 1
1
mins
5 10 20 25 30 35 40 45
5 10 15 20 35 45 min
Lapatinib Anticancer Drug in Human Plasma by LC-MS/MS
1 2
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Column: ACE 5 C18
Dimensions: 100 x 4.6 mm
Part Number: ACE-121-1046
Mobile Phase: 10 mM ammonium formate
pH 3.5/MeCN (10:90 v/v)
Flow Rate: 1 mL/min
Injection: 0.5 μL
Temperature: 40 °C
Detection: API 4000 triple quad MS
Positive ion mode ESI
Ion spray voltage: 5500 V
Temperature: 400 °C
Sample: Extracted from 100 μL plasma
using liquid-liquid extraction
Application #AN3360
Conditions Analytes
1. Lapatinib
(m/z 581.1 365.2)
Concentration 1000 ng/mL
2. Lapatinib-d4 (IS)
(m/z 585.1 365.0)
Concentration 100 ng/mL
Lapatinib Lapatinib-d4 (IS)
45
Time - Minutes
Time - Minutes Time - Minutes
Time 1
Time 2
Time 3
LLOQ 2.5 ng/mL
LOD 1.0 ng/mL
Method Linearity 2.5 – 2500 ng/mL
Reproduced with permission of Department of Chemistry, School of Science, Gujarat University, India