ACE UHPLC and HPLC Columns
Itraconazole and Hydroxyitraconazole in Human Whole Blood by LC-MS/MS
90
Itraconazole Itraconazole-d5
cps cps
250
200
150
100
50
120
80
0
250
250
200
200
150
150
100
100
50
50
120
80
80
40
0
Time - Minutes Time - Minutes
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1.6e5
1.2e5
1.2e5
8.0e4
8.0e4
4.0e4
4.0e4
0.0
0.0
6.0e4
6.0e4
4.0e4
4.0e4
2.0e4
2.0e4
cps
1.2e5
0.0
6.0e4
4.0e4
0.0 0.4 0.8 1.2 1.6 2.0
0.4 0.8 1.2 1.6 2.0
0.0
0.0
0.0 0.4 0.8 1.2 1.6 2.0
40
1 2
3 4
0
1.6e5
0.0
mins mins
Column: ACE 3 C18-AR
Dimensions: 50 x 2.1 mm
Part Number: ACE-119-0502
Mobile Phase: A: 10 mM ammonium
acetate in H2O
B: 10 mM ammonium
acetate in MeOH
Gradient: Time (mins) %B
0 75
2 98
3 98
Flow Rate: 0.7 mL/min
Temperature: 45 °C
Detection: AB Sciex 4000
ESI positive ion mode
Sample: 1.0 ng/mL human whole blood (LLOQ)
Application #AN3380
Conditions Analytes
1. Itraconazole
(m/z 705.3 392.3)
2. Itraconazole-d5
(m/z 710.4 397.4)
3. Hydroxyitraconazole
(m/z 721.3 408.2)
4. Hydroxyitraconazole-d5
(m/z 726.4 413.3)
8.0e4
4.0e4
2.0e4
0.0 0.4 0.8 1.2 1.6 2.0
0.0 0.4 0.8 1.2 1.6 2.0
40
1 2
3 4
0
1.6e5
0.0
mins mins
Hydroxyitraconazole
Hydroxyitraconazole-d5
cps 1 cps 2
0
0
0.0 0.8 2.0
mins mins
Reproduced with permission of Agilux Laboratories, USA
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