ACE UHPLC and HPLC Columns
Isoflavones
300
250
200
150
100
50
0
Isoflavones in Red Clover and Soy Extract
1
2
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Insulins
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: A: 0.1% TFA in H2O/MeCN
(71:29 v/v)
B: 0.1% TFA in H2O/MeCN
(68:32 v/v)
Gradient: Time (mins) %B
0 10
16 90
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 215 nm
Application #AN2770
Conditions Analytes
1. Bovine insulin
2. Human insulin
3. Porcine insulin
1
2
3
2 4 6 8 10 12 14 16 18
Time - Minutes
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: MeCN/0.1% formic acid
in H2O (35:65 v/v)
Flow Rate: 1 mL/min
Injection: 1 μL
Temperature: Ambient
Detection: UV, 254 nm
Application #AN2970
Conditions Analytes
1. Daidzin
2. Genistin
3. Daidzein
4. Genistein
1
2
0 1 2 3 4 6 7 8 9
Time - Minutes
4
5 10
0
Column: ACE 3 C18-AR
Dimensions: 150 x 2.1 mm
Part Number: ACE-119-1502
Mobile Phase: A: Acetic acid in H2O pH 2.8
B: 0.6% Acetic acid in MeCN
Gradient: Time (mins) %B
0 15
7 15
27 75
Flow Rate: 0.35 mL/min
Injection: 3 μL
Temperature: 25 °C
Detection: UV, 254 nm
Application #AN1130
Conditions Analytes
1. Daidzin
2. Glycitin
3. Rutin (Int. Standard)
4. Genistin
5. Acetyldaidzin
6. Acetylglycitin
7. Daidzein
8. Glycitein
9. Acetylgenistin
10. Genistein
11. Formononetin
12. Biochanin A
2
1
3
4 5 6
7
89
10
11
12
Time- Minutes
K. Weinfurter et al. Forsch. Komplementmed. 21 (Suppl.1): 45 (2014)
Response - MilliVolts
Response - MilliVolts
Red clover is a perennial herb that
commonly grows wild in meadows
throughout Europe and Asia.
4
3