ACE UHPLC and HPLC Columns
Illegal Dyes in Spices
Column: ACE 3 C18
Dimensions: 100 x 4.6 mm
Part Number: ACE-111-1046
Mobile Phase: A: H2O
B: MeOH
C: 0.06 M Tetrabutylammonium
bromide and 0.5 M KH2PO4
in H2O pH 2.55
Gradient: Time (mins) %A %B %C Curve
0 45 50 5
45 3 92 5 6
65 3 92 5 11
66 45 50 5 1
75 45 50 5 1
Flow Rate: 1 mL/min
Injection: 10 μL
Temperature: Ambient
Detection: UV-Vis, 420 nm, 520 nm and 600 nm
Insulin Analogues in Clinical and Post-Mortem Analyses
88
3. Aspart
MW 5826
Quantifier (m/z 971.7 136)
Qualifier (m/z 1166 219)
Qualifier (m/z 971.7 226.1)
4. Lispro
MW 5808
Quantifier (m/z 1162.4 217)
Qualifier (m/z 968.6 217)
XIC of +MRM (16 pairs): 1184.000/454.400 Da ID: Detemir 1 from Sample 18 (100uU/mL Mix) of 140716 Insulin Precision + Samples.wiff... Max. 4.5e5 cps.
3,4,5
3,4,5
Aspart
2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0
4.4e5
4.2e5
4.2e5
4.0e5
3.8e5
3.8e5
3.6e5
3.4e5
3.4e5
3.2e5
3.0e5
3.0e5
2.8e5
2.6e5
2.6e5
2.4e5
2.2e5
2.2e5
2.0e5
1.8e5
1.8e5
1.6e5
1.4e5
1.4e5
1.2e5
1.0e5
1.0e5
8.0e4
6.0e4
6.0e4
4.0e4
2.0e4
2.0e4
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Application #AN2910
Conditions Analytes
1. Rhodamine B
2. Orange II
3. Metanil Yellow
4. Butter Yellow
5. Para Red
6. Sudan Orange G
7. Toluidine Red
8. Sudan I
9. Sudan Red G
10. Sudan II
11. Sudan Black
12. Sudan III
13. Sudan Red 7B
14. Sudan Red B
15. Sudan IV
1
2
3
4
5
7/8
6
9
10
11
12
13
14
15
0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68
Reproduced with permission of Lincolne Sutton & Wood, Norwich, UK
Column: ACE 5 C18-300
Dimensions: 50 x 2.1 mm
Part Number: ACE-221-0502
Mobile Phase: A: 0.1% acetic acid in H2O
B: 0.1% acetic acid in
IPA/MeCN (25:75 v/v)
Gradient: Time (mins) %B
0.0 22
0.5 22
1.0 34
3.0 36
4.0 98
6.2 98
6.3 22
Flow Rate: 0.55 mL/min
Injection: 40 μL
Detection: AB Sciex QTRAP 5500
ESI positive ion mode
Ion spray voltage: 5500 V
Temperature: 600 °C
Sample: 100 μU/mL insulin analogues
in steroid-free serum
Application #AN3350
Conditions Analytes
1. Glargine
MW 6063
Quantifier (m/z 867.2 136)
Qualifier (m/z 1011.4 1164.2)
Qualifier (m/z 1011.4 1179.4)
2. Bovine insulin (IS)
MW~5800
Quantifier (m/z 956.5 136.1)
Qualifier (m/z N/A)
Reproduced with permission of St Paul’s Hospital, University of British Columbia, Vancouver, Canada
Time, min
0.0
I n t e n s i t y , c p s
4.75
Printing Time: 8:40:19 AM
Printing Date: December-09-15
Collected by: 5500Q\5500
Electronic Signature: no
Operator: 5500
2
6
1
2
6
0.0
2.4 2.8 3.2 3.6 4.0 4.4 4.8
Intensity, cps
5. Insulin R
MW 5808
Quantifier (m/z 1162.4 345.2)
Qualifier (m/z 1162.3 65.2)
Qualifier (m/z 1162.4 226.1)
6. Detemir
MW 5917
Quantifier (m/z 1184 454.4)
Qualifier (m/z 987 454.4)
Qualifier (m/z 1184.0 357.4)
Time- Minutes
Time - Minutes
a-chain
b-chain
Insulin R
Glargine Lispro
Detemir