ACE UHPLC and HPLC Columns
Herbicide Impurity Profile
Conditions
84
Technical Grade Herbicide
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Application #AN2130
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: CORE-25A-1546U
Mobile Phase: A: MeCN/H2O/TFA (5:95:0.05 v/v/v)
B: MeCN/TFA (99.9:0.05 v/v)
Gradient: Time (mins) %B
0 10
3 10
35 100
55 100
56 10
60 10
Flow Rate: 0.6 mL/min
Injection: 10 μL
Temperature: 25 °C
Detection: UV, 240 nm
Hippuric Acid
Conditions
Application #AN2760
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 10 mM KH2PO4 pH 3.5 in
H2O/MeCN (15:85 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 254 nm
Analytes
1. Hippuric acid
2. 2-Methylhippuric acid
Time - Minutes
Time - Minutes
1
2
Active component
!
Hippuric acid Please enquire for details of
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2-Methylhippuric acid email: info@ace-hplc.com
Response - MilliVolts