ACE UHPLC and HPLC Columns
Hepcidin-25 and Truncated Isoforms by LC-HRMS
Conditions
Analyte
1. Hepcidin-25
(MW 2789)
EIC for hepcidin-25 and truncated isoforms
Herbicide – Benfluralin Herbicide – Trifluralin
Conditions Conditions
Analyte Analyte
1. Benfluralin 1. Trifluralin
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Application #AN3090
Column: ACE 3 C18
Dimensions: 100 x 2.1 mm
Part Number: ACE-111-1002
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0 15
5 100
6 100
Flow Rate: 0.25 mL/min
Injection: 100 μL
Temperature: 60 °C
Detection: ThermoFisher Scientific Q-Exactive™ high resolution MS
Heated electrospray ionisation (positive mode)
Spray voltage: 4.5 kV
Vaporiser temperature: 200 °C
Capillary temperature: 320 °C
Detection: Full scan m/z 400 – 1000
Extracted ion chromatogram from sum of 6 most abundant
isotopes of +3, +4 and +5 charge states
Time - Minutes
Reproduced with permission of Viapath, Toxicology Unit, King’s College Hospital London, UK
Time - Minutes
Application #AN2880 Application #AN2890
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: H2O/MeOH (15:85 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 254 nm
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: H2O/MeOH (15:85 v/v)
Flow Rate: 1 mL/min
Temperature: Ambient
Detection: UV, 254 nm
1
Time - Minutes
1
Hepcidin-25
Hepcidin-20
Hepcidin-22
Relative abundance (%)
Relative abundance (%)
Hepcidin-24
Hepcidin-25
Hepcidin-25-13C18,15N3
Mass Spectrum of hepcidin-25
Response - MilliVolts