ACE UHPLC and HPLC Columns
Gliotoxin from Aspergillus Fumigatus
Liquid Culture
Application #AN3780
Conditions Analyte
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: A: 0.05% TFA in H2O/MeCN (90:10 v/v)
B: 0.05% TFA in H2O/MeCN (40:60 v/v)
Gradient: Time (mins) %B
0 10
21 100
Flow Rate: 1 mL/min
Detection: UV, 254 nm
mAU
80
60
40
20
Glyphosate and Related Compounds as FMOC
Derivatives (Isocratic) Application #AN3860
Conditions Analytes
Column: ACE Excel 3 SuperC18
Dimensions: 150 x 2.1 mm
Part Number: EXL -1111-1502U
Mobile Phase: 5 mM ammonium formate pH
3.0 in H2O/MeOH (55:45 v/v)
Flow Rate: 0.4 mL/min
Injection: 0.1 μL
Temperature: 25 °C
Detection: UV, 254 nm
Sample: Standards derivatised with FMOC-Cl
5
4
3
2
1
80
1
9 11 13 15 17 19 21 23
Time - Minutes
1. Gliotoxin
25
20
15
10
5
1. Glyphosate
2. Aminomethylphosphonic
acid (AMPA)
3. Glufosinate
50
40
30
20
10
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0
1
Svahn KS, Goransson U, Chryssanthou E, Olsen B, Sjolin J, Stromstedt A.
Induction of Gliotoxin Secretion in Aspergillus fumigatus by Bacteria-Associated
Molecules. PloS ONE 9(4): e93685. doi:10.1371/journal.pone.0093685
Glyphosate and Related Compounds as
FMOC Derivatives (Gradient)
Time - Minutes
Application #AN3850
Conditions Analytes
1. Glyphosate
2. Aminomethylphosphonic
acid (AMPA)
3. Glufosinate
Column: ACE Excel 3 SuperC18
Dimensions: 150 x 2.1 mm
Part Number: EXL -1111-1502U
Mobile Phase: A: H2O
B: MeOH
C: 200 mM ammonium formate pH 3.0
Gradient: Time (mins) %A %B %C
0 62.5 35.0 2.5
10 2.5 95.0 2.5
11 2.5 95.0 2.5
12 62.5 35.0 2.5
22 62.5 35.0 2.5
Flow Rate: 0.4 mL/min
Injection: 0.1 μL
Temperature: 60 °C
Detection: UV, 254 nm
Sample: Standards derivatised with FMOC-Cl
Aminomethylphosphonic acid (AMPA)
2 4 6 8 10 min
mAU
0
1
2
3
*FMOC
Glyphosate
Glufosinate
*FMOC
5 10 15 20 25 30 min
mAU
0
1
2
3
Time - Minutes
Aminomethylphosphonic acid (AMPA)
1
2
3
Glyphosate
Glufosinate
Green Tea Extract
Conditions
Time - Minutes
Application #AN4280
Column: ACE Excel 1.7 SuperC18
Dimensions: 100 x 3.0 mm
Part Number: EXL -1711-1003U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0 5
15 20
17 95
18 95
20 5
27 5
Flow Rate: 0.8 mL/min
Injection: 2 μL
Temperature: 80 °C
Detection: UV, 260 nm
Sample: Tablet ground to fine powder and extracted with MeCN/
H2O (1:1 v/v) with ultrasonication. Supernatant diluted with
H2O and filtered using Whatman Mini-Uniprep syringeless filter
System: Chromaster Ultra Rs
0 2 4 6 8 10 12 14 min
mAU
0
mAU