ACE UHPLC and HPLC Columns
Ginkgo Biloba – Ultra Resolution
Conditions
Column: ACE Excel 1.7 C18-PFP
Dimensions: 2 x 100 x 3.0 mm (coupled)
Part Number: 2 x EXL -1710-1003U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0.00 5
0.72 5
15.72 50
18.72 100
20.72 100
22.72 5
Flow Rate: 0.8 mL/min
Injection: 2 μL
Temperature: 80 °C
Detection: UV, 254 nm
Sample: Extract of Ginkgo Biloba
30
25
20
15
10
78
Application #AN2270
2 4 6 8 10 12 min
Time - Minutes
Ginsenoside Rg1
3.0e6
2.0e6
1.0e6
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mAU
5
0
-5
1
2
3
6
4
5
7
8
9
11
12
13
Time - Minutes
10
Ginsenosides from Chinese Medicine by UHPLC-MS/MS
Application #AN3540
Conditions Analytes
1. Ginsenoside Re
(m/z 991 945)
2. Ginsenoside Rg1
(m/z 845 799)
3. Ginsenoside Rf
(m/z 845 799)
4. Ginsenoside Rb1
(m/z 1153 1107)
5. Ginsenoside Rc
(m/z 1123 1077)
6. Ginsenoside Ro
(m/z 1001 955)
7. Ginsenoside Rb2
(m/z 1123 1077)
8. Ginsenoside Rg2
(m/z 829 783)
9. Ginsenoside Rh1
(m/z 683 637)
10. Ginsenoside Rd
(m/z 991 945)
11. Ginsenoside F1
(m/z 683 637)
12. Ginsenoside F2
(m/z 829 783)
13. Ginsenoside Rg3
(m/z 829 783)
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 150 x 3.0 mm
Part Number: CORE-25A-1503U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins) %B
0 25
13 60
15 95
17 95
Flow Rate: 0.4 mL/min
Injection: 2 μL
Temperature: 45 °C
Detection: AB SCIEX 5500 Qtrap MS
ESI in negative ion mode
Source temperature: 450 °C
Sprayer voltage: -4500 V
Stepwise MRM mode for
M + HCOO- > M – H- ion transitions
Mass range 501 – 1250 u (step size 2 u)
Reproduced with permission of Beijing University of Chinese Medicine, Beijing, China
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Ginkgo Biloba - Used in traditional
medicine and as a source of food
0.0
2 4 6 8 10 12 14
Intensity, cps