ACE UHPLC and HPLC Columns
Column: ACE 3 C18
Dimensions: 30 x 4.6 mm
Part Number: ACE-111-0346
Mobile Phase: A: 6.5 mM ammonium acetate in H2O
B: MeCN
C: MeOH
Gradient: Time (mins) %A %B %C
0.0 80 10 10
5.2 0 50 50
5.6 0 0 100
Flow Rate: 2 mL/min
Temperature: 60 °C
Detection: UV, 200-450 nm
Conditions
Column: ACE 3 C18
Dimensions: 50 x 4.6 mm
Part Number: ACE-111-0546
Mobile Phase: H2O/MeCN/TFA
(64:34:0.5 v/v/v)
Flow Rate: 2 mL/min
Injection: 20 μL
Temperature: 28 °C
Detection: UV, 254 nm
76
1. Rutin
2. Quercetin
3. Quercitrin
4. Kaempferol
Conditions
Column: ACE Excel 2 C18-AR
Dimensions: 50 x 2.1 mm
Part Number: EXL -109-0502U
Mobile Phase: A: 0.02% formic acid in H2O
B: 0.02% formic acid in H2O/MeOH (2:98 v/v)
Gradient: Time (mins) %B
0.00 10
0.20 10
2.00 40
2.01 100
3.50 100
3.51 10
4.00 10
Flow Rate: 0.75 mL/min
Temperature: 60 °C
Detection: AB SCIEX QTRAP 5500 LC-MS/MS system
Sample: Extracted by mixed mode cation exchange SPE
800
700
600
500
400
300
200
100
0
Application #AN3630
2
Analytes
1. 2-(2-Fluoro-4-biphenyl)-2-
hydroxypropionic acid
2. cis-2-(2-Fluoro-4-biphenyl)-2-
hydroxypropionic acid
3. 2-Fluoro-4-biphenyl-4-carboxylic acid
4. Flurbiprofen
(2-(2-Fluorobiphenyl-4-yl)propionic acid)
5. 4-Acetyl-2-fluorobiphenyl
5
Time - Minutes Time - Minutes
www.ace-hplc.com | +44 (0) 1224 704 554 | info@ace-hplc.com
0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8
Flurbiprofen and Related Substances
Reproduced with permission of School of Pharmacy, University of Sunderland, UK
Flavonoids
Conditions
Application #AN2810
Analytes
Application #AN3100
Analyte
1. Formoterol
(m/z 345 149)
Column: ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: MeCN/0.1% formic
acid in H2O (40:60 v/v)
Flow Rate: 1 mL/min
Injection: 1 μL
Temperature: Ambient
Detection: UV, 254 nm
1
4
2, 3
1
1 2
0 2
3
4
4 6 8 10 12 14
Formoterol from Human Plasma by LC-MS/MS
Reproduced with permission of Bioanalytical Systems, Inc
Flavone and Dibucaine
Time - Minutes
Application #AN2850
Conditions Analytes
1. Flavone
2. Dibucaine
Time - Minutes
Flavone
Dibucaine
Rutin
Quercetin
Quercitrin
Kaempferol
Reproduced with permission of Johnson & Johnson Pharmaceutical Research
& Early Development, a division of Janssen-Cilag, S.A, Toledo, Spain
Typical LLOQ standard
calibrator of 0.5 pg/mL
Formoterol
Flurbiprofen
Intensity cps Response - MilliVolts