ACE UHPLC and HPLC Columns
Conditions Analytes
1. Degradation Product
2. Entacapone
2
Entacapone
0.12
0.1
0.08
0.06
0.04
0.02
Reproduced with permission of Brazilian Pharmacopoeia Reproduced with permission of School of Pharmacy, University of Sunderland, UK
Application #AN3620
Conditions
Column: ACE 5 C18
Dimesions 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: A: MeOH
B: H2O
Gradient: Time (mins) %B
0.0 85
2.5 85
60.0 50
62.5 50
70.0 85
Flow Rate: 2.0 mL/min
Temperature: Ambient
Detection: UV, 230 nm
0 10 20 30 40 50 60
1
Column: ACE 5 C18
Dimensions: 250 x 4.6 mm
Part Number: ACE-121-2546
Mobile Phase: Phosphoric acid pH 3.0/MeCN
(65:35 v/v)
Flow Rate: 2.0 mL/min
Injection: 20 μL
Temperature: 25 °C
Detection: UV, 305 nm
Sample: Entacapone standard in
MeOH solution exposed to
direct UV radiation (254 nm)
Time - Minutes Time - Minutes
Application #AN2900
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60
Column: ACE 5 C18 1. Epinastine
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: 0.3% TEA pH 4.0 with
phosphoric acid/MeOH (60:40 v/v)
Flow Rate: 1 mL/min
Injection: 20 μL
Temperature: 25 °C
Detection: UV, 254 nm
B
Conditions
A
B
0 2 4 6 8 10
B
A
mins
0 2 4 6 8 10
Analyte
B
A
Magnified
Epinastine
Reproduced with permission of Brazilian Pharmacopoeia
Epanolol
Conditions
Application #AN3610
Analyte
Column: ACE 5 CN 1. Epanolol
Dimensions: 150 x 4.6 mm
Part Number: ACE-124-1546
Mobile Phase: 20 mM ammonium formate
pH 3.0/MeOH (85:15 v/v)
Flow Rate: 1 mL/min
Injection: 20 μL
Temperature: Ambient
Detection: UV, 254 nm
1
Ethanol Extract from Seed Cover
(Acacia Farnesiana)
Reproduced with permission of School of Pharmacy, University of Sunderland, UK
Entacapone
Time - Minutes
Application #AN3600
0 1 2 3 4 5 6 7 8 9
50
40
30
20
10
0
Time - Minutes
(A) Epinastine Hydrochloride
before UV radiation
(B) Epinastine Hydrochloride
after 18 hours UV radiation
0
0
1 2 3 4 5 6 7 8 9
Magnified
mAU