ACE UHPLC and HPLC Columns
Echinacea
Conditions
250
250
200
200
150
150
100
100
50
mAU
200
200
150
150
100
100
0 5 10 15 20 25 30 35 40 45m in
50
50
mAU
200
200
150
150
100
100
50
50
72
0 5 10 15 20 25 30 35 40 45min
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mAU
0
0 5 10 15 20 25 30 35 40 45m in
0
Rs: 1.2
Rs: 1.68
Rs: 2.06
mAU
0
45 min
mAU
0
0 5 10 15 20 25 30 35 40 45 min
0
0 5 10 15 20 25 30 35 40 45 min
mAU
0
Application #AN4270
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 150 x 3.0 mm; 2 x 150 x 3.0 mm (coupled); 3 x 150 x 3.0 mm (coupled)
Part Number: CORE-25A-1503U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient: Time (mins)
150 x 3.0 mm 300 x 3.0 mm 450 x 3.0 mm %B
- 0.00 0.00 5
0.00 0.47 0.94 5
20.00 40.47 60.94 100
25.00 45.47 75.94 100
26.00 46.47 76.94 5
46.00 86.47 136.94 5
Flow Rate: 0.43 mL/min
Temperature: 80 °C
Detection: UV, 254 nm
150 x 3.0 mm
300 x 3.0 mm
450 x 3.0 mm
Time - Minutes
Rs: 1.2
Rs: 1.68
Rs: 2.06
