ACE UHPLC and HPLC Columns
Drugs of Abuse Screen (250 Analytes) in Urine by LC-MS/MS
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Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL -1010-1002U
Mobile Phase: A: 2 mM ammonium acetate + 0.1%
formic acid in H2O
B: 2 mM ammonium acetate + 0.1%
formic acid in MeOH
Gradient: Time (mins) %B
0 2
4 2
34 100
38 100
40 2
Flow Rate: 0.3 mL/min
Injection: 10 μL
Temperature: 37 °C
Detection: Thermo Quantum Ultra MS
ESI in positive ion mode
Analytes in blue are included in
Extracted Ion Chromatogram
Application #AN4140
Conditions
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00 55 1100 1155 2200 2255 3300
0
Reproduced with permission of Toxicology, Clinical Chemistry, Wansbeck General Hospital, Northumberland, UK
Analyte Rt (Mins) MRM
Transitions (m/z)
Analyte Rt (Mins) MRM
Transitions (m/z)
6-Acetylcodeine 16.9 342.2 > 165.1 Buphedrone 14.2 178.2 > 160.1
Alfentanil 21.1 417.4 > 197.2 Buphedrone ephedrine 13.5 180.4 > 162.2
Alprazolam 23.3 309.11 > 205.1 Bupivacaine 19.5 289.2 > 84.2
Aminoclonazepam 13.9 286.2 > 222.3 Buprenorphine glucuronide 18.1 644.4 > 468.4
Aminodesmethylflunitrazepam 20.0 270.1 > 121.1 Bupropion 19.5 240.1 > 131.1
Aminoflunitrazepam 16.2 284.1 > 135.1 Butylone 14.7 222.1 > 131.1
Aminonitrazepam 10.9 252.1 > 94.1 Caffeine 13.1 195.1 > 110.1
Aminorex 12.2 163 > 120.1 Camfetamine 18.7 202.2 > 67.1
Amiodarone 31.3 646.2 > 100 Carbamazepine 21.5 237.1 > 192.2
Amisulpride 16.2 370.1 > 242 Carbamazepine 10,11-Epoxide 18.6 253.2 > 180.1
Amitriptyline 24.4 278.2 > 91.1 Cathine 20.0 134.2 > 91.1
Amlodipine 25.3 409.3 > 238.2 Cathinone 9.5 150.2 > 117.1
Amlodipine met 22.9 407 > 318 2C-B-FLY 20.1 286 > 269.1
Amphetamine 11.6 136.1 > 65.2 Chlordiazepoxide 19.5 300.1 > 227.1
Amphetamine-d6 11.6 142.2 > 67.2 Chloroquine 18.5 320.1 > 142.1
Anhydroecgonine methyl ester 9.8 182.2 > 91.1 Chlorpheniramine 20.4 275.1 > 167.2
Aripiprazole 25.5 448.1 > 285.1 Chlorpromazine 25.9 319.2 > 58.1
Atenolol 10.7 267.1 >145.1 Citalopram 22.2 325.1 > 246.1
Atomoxetine 23.1 256.2 > 44.1 Clobazam 22.8 301.1 > 259.2
Atomoxetine metabolite 22.5 242.2 > 44.1 Clomethiazole 17.3 161.9 > 113
Benzedrone 21.7 254 > 65.1 Clomipramine 27.0 315.1 > 86.1
Benzoylecgonine 15.6 290.1 > 77.2 Clonazepam 23.1 316.1 > 214.2
Benzoylecgonine-d3 15.6 293.2 > 77.2 Clonidine 13.2 230 > 44.2
Benzylpiperazine 5.1 177.2 > 65.1 Clozapine 21.7 327.1 > 192.1
Bisoprolol 19.9 326.3 > 116.1 Cocaethylene 19.5 318.2 > 82.2
Bromazepam 20.4 316 > 182.1 Cocaine 18.0 304.2 > 82.2