ACE UHPLC and HPLC Columns
Drugs of Abuse Screen by UHPLC-MS/MS
Column: ACE Excel 1.7 C18
Dimensions: 100 x 2.1 mm
Part Number: EXL -171-1002U
Mobile Phase: A: 5 mM ammonium acetate in H2O
B: 5 mM ammonium acetate in MeOH
Gradient: Time (mins) %B
0.0 10
10.0 90
11.9 90
13.4 10
15.5 10
Flow Rate: 0.3 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: MS Quattro Premier XE triple quad
MRM, positive and negative ESI mode
Desolvation temperature: 450 °C
Ion source temperature: 150 °C
Collision gas pressure: 3.5 x 10-3 mbar
68
5
6
7
14
12
15
16
100
%
0
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Application #AN2190
Conditions
Reproduced with permission of Biotage GB Ltd, UK
1
2
3
4
8
9
10
11
13
17
18
19
20
21
35
24
23
22
28
25 26,27
29
31
34
32
30
36
33
37,38,39
41
40
42
46,47
45
44
43
49,50
51
48
2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00
Time - Minutes
Peak Analyte Rt (Mins) Q1 (Da) Q3 (Da) Peak Analyte Rt (Mins) Q1 (Da) Q3 (Da)
1 Oxymorphone 2.62 302.2 198.1 27 Bromazepam 6.95 316.1 182.1
2 Morphine-d3 2.82 289.2 201.0 28 Ketamine 7.21 238.1 124.9
3 Morphine 2.95 286.2 201.0 29 Clonazepam 7.26 316.1 270.1
4 Hydromorphone 3.25 286.2 185.1 30 Nitrazepam 7.26 282.2 236.1
5 Amphetamine-d5 3.62 141.0 123.9 31 α-Hydroxytriazolam 7.34 359.1 331.1
6 Amphetamine 3.65 136.0 118.9 32 Flunitrazepam 7.34 314.2 268.2
7 Dihydrocodeine 3.66 302.2 199.1 33 α-Hydroxyalprazolam 7.54 325.2 297.1
8 MDA 3.67 180.1 105.0 34 Estazolam 7.56 295.2 267.2
9 MDMA 3.75 194.1 163.0 35 Zolpidem 7.73 308.2 235.1
10 Methamphetamine 3.80 150.0 90.9 36 Triazolam 7.77 343.0 308.1
11 Oxycodone 4.03 316.2 241.2 37 2-Hydroxyethylflurazepam 7.77 333.2 109.0
12 MDEA 4.12 208.2 163.0 38 Lorazepam 7.80 321.1 275.1
13 BZE-d3 4.15 293.1 171.0 39 Oxazepam 7.82 287.2 241.0
14 BZE 4.17 290.1 168.0 40 Alprazolam 7.85 309.2 281.2
15 6-MAM 4.48 328.2 165.1 41 Methadone 7.99 310.2 265.2
16 Codeine 4.59 300.3 215.1 42 Temazepam 8.05 301.1 255.1
17 Norfentanyl 4.71 233.1 84.0 43 Nordiazepam 8.44 271.1 139.9
18 7-Amino-clonazepam 4.77 286.2 121.0 44 Midazolam 8.61 326.2 291.2
19 Hydrocodone 4.94 300.2 199.1 45 Diazepam-d5 8.63 290.2 154.0
20 7-Amino-flunitrazepam 5.34 284.2 135.0 46 Diazepam 8.67 285.2 154.0
21 Cocaine 5.99 304.2 182.0 47 Flurazepam 8.68 388.2 315.1
22 Norbuprenorphine 6.47 414.3 101.0 48 Fentanyl 8.85 337.3 105.0
23 PCP 6.49 244.2 159.9 49 THC-COOH-d3 9.98 348.2 302.2
24 Zaleplon 6.55 306.2 264.2 50 THC-COOH 10.01 345.2 299.2
25 EDDP 6.58 278.2 234.2 51 Buprenorphine 11.70 468.3 101.0
26 Norketamine 6.90 224.1 124.9