ACE UHPLC and HPLC Columns
Dermorphin in Equine Urine by LC-MS/MS
1
1.00
0.75
0.50
0.25
0.00
Accurate quantification of dermorphin in equine urine
in range 0.05 – 100 ng/mL
600
LLOQ = 0.05 ng/mL 2
500
400
300
200
100
Diuretics (Isocratic)
I0
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Column: ACE 3 C18
Dimensions: 100 x 2.1 mm
Part Number: ACE-111-1002
Mobile Phase: A: 0.2% formic acid in H2O
B: 0.2% formic acid in MeCN
Gradient: Time (mins) %B
0.00 5
0.20 5
8.00 95
8.50 95
8.51 5
12.50 5
Flow Rate: 0.4 mL/min
Injection: 40 μL
Detection: Bruker EVOQ Elite triple quad MS
VIP heated-ESI temperature: 350 °C
Cone gas temperature: 250 °C
Spray voltage: +4000 V
Application #AN1040
Conditions Analytes
1. Dermorphin
(m/z 803.4 602 (Quantifier ion))
(m/z 803.4 202 (Qualifier ion))
2. Deltorphin-II (IS)
(m/z 783 277)
1 2 3 4 5
0
Reproduced with permission of Bruker UK Ltd (Bruker Application Note #704423)
Dermorphin
Deltorphin-II (IS)
Didanosine
Column: ACE 5 C18-HL
Dimensions: 250 x 4.6 mm
Part Number: ACE-321-2546
Mobile Phase: 50 mM ammonium acetate
pH 8.0/MeOH (80:20 v/v)
Flow Rate: 1.5 mL/min
Temperature: Ambient
Detection: UV, 254 nm
Application #AN3590
Conditions Analyte
1. Didanosine
1
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
Time - Minutes
Time - Minutes
3.5
3.0
2.5
2.0
1.5
1.0
0.5
0.0
Reproduced with permission of Aptuit Ltd, Edinburgh, UK
6
Column: ACE Excel 2 C18-PFP
Dimensions: 50 x 3.0 mm
Part Number: EXL -1010-0503U
Mobile Phase: 10 mM ammonium formate
pH 3.0 in MeOH/H2O (45:55 v/v)
Flow Rate: 1 mL/min
Injection: 2 μL
Temperature: 60 °C
Detection: UV, 254 nm
Application #AN2140
Conditions Analytes
1. Furosemide
2. Indapamide
3. Bendroflumethiazide
4. Spironolactone
1
2
3
4
0 11 22 33 44 55 mminins
0 1 2 3 4 5
Furosemide
Indapamide
Bendroflumethiazide
Spironolactone
Time - Minutes
Response -
MilliVolts
kCps kCps